Study of the microbiome in animal sciences: methodological and applicative aspects

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Coordinator : Pulina Giuseppe


Members:

Marcello Mele, Anna Nudda, Bruno Stefanon, Luciano Pinotti, Valentino Palombo, Achille Schiavone, Massimo Trabalza Marinucci, Rosanna Marino

Raffaella Tudisco, Bianca Castiglioni, Genciana Terova, Anna De Angelis, Giuseppe Moniello

Mariano Pauselli, Antonella Chiariotti, Luciana Rossi, Alberto Palmonari


Targets:

  • draw up and administer a questionnaire concerning the collection of analytical approaches for the analysis of the microbiome, availability of microbial biobanks or biorepository of biological matrices characterizing the microbial biodiversity of the national zootechnical landscape

  • mapping of sequencing infrastructures dedicated to animal husbandry (real dimensioning of sequencing needs)

  • mapping skills of analysis of 16s rRNA, metagenome and "omics" data in general


Activities:
 

  • State of the art on the knowledge of the diversity and functions of the microbiome of the different species of zootechnical interest

  • Deepening and discussion of:

  1. methodological aspects: experimental designs adopted in in vitro and in vivo trials

  2. sampling methods used for the collection of biological samples to be used for the study of the microbiome and for their conservation

  3. methodologies adopted for the investigation of the microbiome according to the objectives of the study

  4. Bioinformatic and biostatistic analytical approaches, development of complex models that integrate microbiome data and other descriptive covariants of the physiological state of animals

     

Deliverable:

  1. Review in scientific journals according to the data collected by the questionnaire

  2. Operational guidelines for sample collection, sample storage and multiomocs data analysis.

  3. Summer school: early stage and advance stage



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